CDK20+Cyclin H NanoBRET Kinase Assay

CDK20+Cyclin H NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview


CDK20+Cyclin H

HGNC Symbol

CDK20/CCNH complex


CAK-kinase p42; CCRK; CDCH; p42; PNQALRE

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-CDK20 Fusion Vector and Cyclin H Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-10 and then treated with reference compound PBI7627 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
PBI7627 6.015e-008

Screening Location

Malvern, USA

Reference compound IC50 for CDK20+Cyclin H