CDK5+CDK5R1

NanoBRET Target Engagement Intracellular Kinase Assay, Competitive binding assay format

Target

CDK5+CDK5R1

HGNC Symbol

CDK5/CDK5R1 complex

Synonyms

PSSALRE

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently co-expressing NanoLuc-CDK5 fusion vector and CDK5R1 Vector were seeded into the wells of 384-well plates. The cells were pretreated with the NanoBRET Tracer K-10 and then treated with reference compound APY-69 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
APY-69 4.507e-009
Screening Location

Malvern, USA

Reference compound IC50 for CDK5+CDK5R1