CDK6+Cyclin D1 NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently co-expressing NanoLuc-CDK6 fusion vector and Cyclin D1 Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-7 and then treated with reference compound Palbociclib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
Malvern, PA, USA
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Reference compound IC50 for CDK6+Cyclin D1