CDK9+Cyclin K NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
C-2k, CDC2L4, PITALRE, TAK
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-CDK9 Fusion Vector and Cyclin K Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound Dinaciclib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Binding of compound to target kinase