Cereblon (Mouse) Binding Assay Service

Mouse Cereblon/hDDB1 binding assay for compound screening and profiling via probe displacement monitored through a change in fluorescence polarization

Target Overview

CAT#

Mouse Cereblon Direct

Enzyme

Mouse Cereblon/hDDB1 complex

Probe

Bodipy-thalidomide

Scientific Information

Cereblon: Q8C7D2 (https://www.uniprot.org/uniprotkb/Q8C7D2/entry)
DDB1: Q16531 (https://www.uniprot.org/uniprotkb/Q16531/entry)

Assay Properties

Reaction

mCereblon/hDDB1 + probe = FP signal

Readout

Change in FP signal upon probe displacement

Reference Compound IC50s

Compounds	IC50 (nM)
Thalidomide	260.8
Lenalidomide	161.9
Pomalidomide	94.3

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Protein Degradation Assay – PROTAC Screening

Reference compound IC50 data for mCereblon/hDDB1

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