Cereblon/DDB1 binding assay for compound screening and profiling via probe displacement monitored through a change in fluorescence polarization
Target Overview
CAT#
Cereblon Direct
Enzyme
Human Cereblon/DDB1 complex
Probe
Bodipy-thalidomide
Scientific Information
Assay Properties
Reaction
Cereblon/DDB1 + probe = FP signal
Readout
Change in FP signal upon probe displacement
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Thalidomide | 347.2 |
| Lenalidomide | 268.6 |
| Pomalidomide | 153.9 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Protein Degradation Assay – PROTAC Screening
Reference compound inhibition of probe binding to Cereblon
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