Surface Plasmon Resonance (SPR) technique for evaluating direct binding of cereblon CULT domain to molecules
Target Overview
Target
CULT Domain of Protein Cereblon (aa 316-442)
HGNC symbol
CRBN
Synonyms
MRT2, MRT2A
Uniprot page
Q96SW2 (https://www.uniprot.org/uniprot/Q96SW2)
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding kinetics of thalidomide and analogues pomalidomide and lenalidomide to the CULT domain of cereblon
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model and steady state affinity model to determine binding affinity (KD).
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