CSNK1E NanoBRET Kinase Assay

CSNK1E NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target. 

Target Overview



HGNC Symbol



CKIe, HCKIE, MGC10398, CK1-epsilon

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-CSNK1E Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-8 and then treated with reference compound AZD-5438 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
AZD-5438 1.732e-006

Screening Location

Malvern, USA

Reference compound IC50 for CSNK1E