Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a key regulator of T-cell immunity by maintaining activation and inhibition of T-cell immune responses. CTLA4 and CD28 are co-stimulatory and co-inhibitory cell surface signaling proteins that interact with the same ligands (CD80 and CD86), with CTLA4 displaying a greater affinity than CD28 for both, thus creating effective ligand binding competition. Studies have shown that functional blockage of CTL4 by anti-CTL4 binding by biologics and small molecules with high affinity results in enhanced T cell responses, ultimately resulting in more effective immune responses targeting many cancers.
To provide a screening assay for identifying small and large molecule CTLA-4 and CD86 binding interaction blockage inhibitors, with the goal of eventually studying immune responses to aid in the discovery of novel immunotherapies to combat cancer.
Human CTLA4, human CD86
CTLA4: Cytotoxic T-lymphocyte protein 4, CD152
CD86: T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, FUN-1, CTLA-4 counter-receptor B7.2, CD28LG2
Binding interaction between CTLA4 and CD86
Malvern, PA, USA
More information can be found on our website Immune Checkpoint Assays
Reference compound inhibition of CTLA4-CD86 binding interaction