CYP19A Inhibition Assay Service

CYP19A for compound screening and profiling via fluorescence-based quantification of enzyme activity.

Target Overview
CAT#

CYP19A

Enzyme

Human CYP19A (recombinant protein)

Synonym

Aromatase, Cytochrome P450 19A1, Estrogen synthase, ARO1, CP19A

Substrate

Fluorescent substrate

Assay Properties
Reaction

Testosterone + 3 [reduced NADPH-hemoprotein reductase] + 3 O₂ = 17-betaestradiol + formate + 4 H₂O + 3 [oxidized NADPH-hemoprotein reductase]

Readout

Enzymatic activity

Reference Compound IC50s
Scientific Information

P11511 (http://www.uniprot.org/uniprot/P11511)

Screening Location

Malvern, USA

Further information

More information can be found on our website Cytochrome P450 Assay Services.

Reference compound IC50 for CYP19A