DHODH Dehydrogenase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
DHODH
Enzyme
Human DHODH
Synonym
Dihydroorotate dehydrogenase (quinone) mitochondrial, DHOdehase, Dihydroorotate oxidase
Substrate
25 µM L-DHO, 60 µM resazurin, Cofactor FMN
Assay Properties
Reaction
L-DHO + FMN = Orotate + FMNH2
FMNH2 + Resazurin = FMN + Resorufin
Resorufin fluorescence, End point measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| BAY-2402234 | 0.42 |
| Brequinar | 2.1 |
| Teriflunomide | 24.5 |
| Leflunomide | 332.9 |
Scientific Information
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Nucleotide Metabolism Pathway Assays
Reference compound inhibition of DHODH
