Nucleotide Metabolism Pathway Assays

Nucleotide metabolism is the process in which nucleic acids (RNA, DNA, and cellular bioenergetics) are synthesized and degraded. DHODH (dihydroorotate dehydrogenase) synthesizes orotate from dihydroorotate (DHO) in the de novo pyrimidine synthesis pathway. The enzyme was shown to indude the differentiation of acute myeloide lymphomas and is therefore interesting as a drug target.

List of Targets

Target HGNC Symbol Synonyms Data
DHODH DHODH dihydroorotate dehydrogenase (quinone)

Additional Information

Assay Setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format. 

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.