EIF2AK2(252-551) NanoBRET Kinase Assay

EIF2AK2(252-551) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview

Target

EIF2AK2(252-551)

HGNC Symbol

EIF2AK2

Synonyms

EIF2AK1, PKR, PRKR, EIF2AK2, PKR

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-EIF2AK2(252-551) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-10 and then treated with reference compound CC1 Pan Kinase Inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.

Readout

Binding of compound to target

Reference Compound IC50s

Compounds	IC50 (M)
CC1 Pan Kinase Inhibitor	1.806e-008

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.

Reference compound IC50 for EIF2AK2(252-551)

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