To test the direct binding to the p300 bromodomain using surface plasmon resonance (SPR) technique
Target Overview
Target
Histone acetyltransferase p300 (Bromodomain), AA 1040-1161
HGNC symbol
EP300
Synonyms
EP300, p300
Uniprot page
Q9BYF1 (https://www.uniprot.org/uniprot/Q9BYF1)
Protein Source
Reaction Biology Cat# RD-11-222 (http://reactionbiology-com.3dcartstores.com/EP300-His_p_83.html)
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
The data was collected on a Biacore 8K using multi-cycle kinetics. Data was collected under reduced DMSO conditions. The data was analyzed using a steady state affinity model to determine binding affinity (KD).
PDF