FGFR2 (M537I) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
FGFR2 (M537I)
FGFR2
BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, KGFR, KSAM, K-SAM, TK14, TK25
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing FGFR2 (M537I)-NanoLuc Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-10 and then treated with reference compound CC1 Pan Kinase Inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target
| Compounds | IC50 (M) |
|---|---|
| CC1 Pan Kinase Inhibitor | 1.667e-008 |
Malvern, PA, USA
More information can be found on our website NanoBRET Target Engagement Intracellular Kinase Assay Services.
