Histone-lysine N-methyltransferase G9a assay with surface plasmon resonance (SPR) format
Histone-lysine N-methyltransferase EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3 (H3-K9-HMTase 3), Lysine N-methyltransferase 1C
UNC0638 binding to G9a in the absence and presence of SAM
The binding of UNC0638 to G9a was measured in the absence and presence of SAM using a Biacore 8K. Including SAM alters the binding kinetics of UNC0638 resulting in an approximately 10-fold slower off-rate and, as a result, an approximately 10-fold tighter KD. Due to the slower off-rate, single-cycle kinetics were used to measure the binding of UNC0638 in the presence of SAM instead of the multi-cycle kinetics used for the measurement in the absence of SAM. For both measurements a 1:1 kinetic binding model was used to fit the data.