Cellular HDAC-Glo Class I/II Assay

Cell-based epigenetic assay for the detection of the relative activity of histone deacetylase (HDAC) class I/II enzymes from cells.

Cell Line

THP-1 human acute monocytic leukemia cell line from ATCC

Assay Format

HDAC-Glo

Assay Protocol

THP-1cells were seeded into the wells of 384-well plate. The cells were treated with reference compound TSA (starting at 1 µM, 10-dose with 3-fold dilution) for 1 hour. HDAC Class I/II activity was measured with HDAC-Glo Class I/II Assay on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose-response equation.

Readout

Measurement for deacetylation of the peptide substrate via luminescence

Reference Compound IC50s
Compound IC50 (µM)
TSA 0.003161

 

Screening location

Malvern, PA, USA

IC50 of TSA in cell-based HDAC-glo Class I/II assay