HDAC6 NanoBRET Target Engagement Intracellular HDAC Assay, competitive binding assay format
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular HDAC Assay
HEK293 cells transiently expressing NanoLuc-HDAC6 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET intracellular Target Engagement HDAC Tracer and then treated with reference compound SAHA for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target HDAC
Malvern, PA, USA
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Reference compound IC50 for HDAC6