Surface Plasmon Resonance (SPR) technique for evaluating direct binding of HRAS to molecular inhibitors
H-Ras (aa 2-169) with a C-terminal biotinylation expressed in E. coli
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Binding of reference compound (BI-2852) to H-RAS
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model to determine binding affinity (KD). Data shown here was collected for "as is" protein assumed to be in the GDP bound state.