K192 Kinase (Kinome-Wide) Panel NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
Target Overview
Target
K192 Kinase (Kinome-Wide) Panel
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Properties
Assay Format
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
Assay Protocol
HEK293 cells transiently expressing each K192 NanoLuc Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with NanoBRET Tracer K-10 and then treated with reference compound CTx-0294885 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. Bar graphs of the normalized BRET responses for K192 kinases were generated using GraphPad Prism program.
Readout
Binding of compound to target
Screening Location
Malvern, PA, USA
K192 Kinase Panel NanoBRET Response
NanoBRET Target Engagement Intracellular K192 Assay Target List
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