K-Ras::RAF1 Protein-Protein Interaction Assay Service (HTRF)

KRAS-related Assays: K-Ras::Raf1 Protein-Protein Interaction Assay Service for compound screening and profiling using the HTRF assay format to measure the binding of K-Ras and Raf1

Target

Human RAF1, human K-Ras

HGNC

RAF1, KRAS

Protein

Kras (aa 2-169), biotinylated, N-terminal his tag. Expressed in E. coli.

cRAF (aa 2-303), N-terminal GST tag. Expressed in E. coli.

Synonyms

RAF1: c-Raf

K-Ras: KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2

Scientific information

RAF1: P04049 (https://www.uniprot.org/uniprot/P04049)

K-Ras: P01116 (https://www.uniprot.org/uniprot/P01116)

Screening location

Malvern, PA, US

Reaction

Binding of K-Ras-GTP to RAF1

Readout

HTRF

KRAS-RAF1_PPI_HTRF_RAS_Malvern