KRAS-related Assays: K-Ras::Raf1 Protein-Protein Interaction Assay Service for compound screening and profiling using the HTRF assay format to measure the binding of K-Ras and Raf1
Target Overview
Target
Human RAF1, human K-Ras
HGNC
RAF1, KRAS
Protein
Kras (aa 2-169), biotinylated, N-terminal his tag. Expressed in E. coli.
cRAF (aa 2-303), N-terminal GST tag. Expressed in E. coli.
Synonyms
RAF1: c-Raf
K-Ras: KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Assay Properties
Scientific information
Screening location
Malvern, PA, US
Reaction
Binding of K-Ras-GTP to RAF1
Readout
HTRF
PDF