K-Ras::SOS1 Protein-Protein Interaction Assay Service (HTRF)

KRAS-related Assays: K-Ras::SOS1 Protein-Protein Interaction Assay Service for compound screening and profiling using the HTRF assay format to measure the binding of K-Ras and SOS1

Target

Human SOS1, human K-Ras

HGNC

SOS1, KRAS

Protein

K-Ras (aa 2-169), biotinylated, untagged. Expressed in E. coli.
SOS1 (aa 564-1069), His/strep tagged. Expressed in E. coli.

Synonyms

SOS1: Son of sevenless homolog 1, GF1, GGF1, GINGF, HGF, NS4

K-Ras: C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, Kras

Scientific information

SOS1: Q07889 (https://www.uniprot.org/uniprot/Q07889)

K-Ras: P01116 (https://www.uniprot.org/uniprot/P01116)

Screening location

Malvern, PA, US

Reaction

Binding of K-Ras-GDP to SOS1

Readout

HTRF

Reference compound EC50s
Compound EC50 (nM)
BAY-293 20

Reference compound BAY-293 inhibits the protein-protein interaction of K-Ras-GDP and SOS1

KRAS-SOS1_PPI_HTRF_RAS_Malvern