KRAS (G12C) NanoBRET Ras Assay

KRAS (G12C) NanoBRET Target Engagement Intracellular Ras Assay for screening of compounds binding to the target.

Target Overview
Target

KRAS (G12C)

HGNC Symbol

KRAS

Synonyms

KRAS2B, KRAS2A, KRAS4A, KRAS4B, RASK2

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties
Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Ras Assay

Assay Protocol

HEK293 cells transiently expressing LgBiT®-KRAS (G12C) and SmBiT®-KRAS (G12C) Fusion Vectors were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-1 and then treated with reference compound BI-2852 for 2 hours. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.

Readout

Binding of compound to target Ras

Reference Compound IC50s
Compounds IC50 (M)
BI-2852 3.873e-007
Screening Location

Malvern, USA

Reference compound IC50 for KRAS (G12C)