KRAS (G12D) NanoBRET Target Engagement Intracellular RAS Assay for screening of compounds binding to the target.
KRAS2B, KRAS2A, KRAS4A, KRAS4B, RASK2
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular RAS Assay
HEK293 cells transiently expressing LgBiT®-KRAS(G12D) and SmBiT®-KRAS(G12D) Fusion Vectors were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET RAS Tracer K-1 and then treated with reference compound MRTX1133 for 2 hours. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target RAS
Malvern, PA, USA
More information can be found on our website RAS Assays for Drug Discovery.
Reference Compound IC50 for KRAS (G12D)