KRAS(G12C) Ternary Complex PROTAC Assay Service

KRAS(G12C) NanoBRET Ternary Complex Formation Assay in HEK293 Cells, Protein Degradation in PROTAC

Target Overview

Target

KRAS(G12C)

HGNC Symbol

KRAS(G12C)

Synonyms

KRAS(G12C)

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using NanoBRET Ternary Complex Formation Assay

Assay Protocol

HEK293 cells transiently co-expressing LgBiT-KRAS(G12C), SmBiT-KRAS(G12C) and HaloTag-CRBN or HaloTag-VHL Fusion Vectors were seeded into the wells of 384-well plates. The cells were treated with reference PROTAC KRAS G12C degrader 1 or LC-2 for 4 hours. The BRET signal was measured on an Envision 2104 Multilabel Reader. BRET ratio was calculated and plotted using the GraphPad Prism program.

Readout

KRAS(G12C)/PROTAC/E3 ligase ternary complex formation

Reference Compounds

PROTAC Compounds
KRAS G12C degrader 1 and LC-2

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Protein Degradation Assay – PROTAC Screening

KRAS(G12C)/CRBN

KRAS(G12C)/VHL

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