MAPK14 (T106M) NanoBRET Kinase Assay

MAPK14 (T106M) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.

Target Overview


MAPK14 (T106M)

HGNC Symbol

MAPK14 (T106M)


p38-alpha, CSBP, CSBP1, p38alpha, p38a

Cell Line

HEK293 human epithelial cell line from ATCC

Assay Properties

Assay Format

384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay

Assay Protocol

HEK293 cells transiently expressing NanoLuc-MAPK14 (T106M) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound CC1-Pan Kinase Inhibitor for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.


Binding of compound to target kinase

Reference Compound IC50s
Compounds IC50 (M)
CC1-Pan kinase Inhibitor 1.58E-08

Screening Location

Malvern, PA, USA

Reference Compound IC50 for MAPK14 (T106M)

Reference compound IC50 for MAPK14 (T106M)