MARK4

NanoBRET Target Engagement Intracellular Kinase Assay, Competitive binding assay format
Target
MARK4
HGNC Symbol
MARK4
Synonyms
KIAA1860; MARKL1
Cell Line
HEK293 human epithelial cell line from ATCC
Assay Format
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
Assay Protocol
HEK293 cells transiently expressing NanoLuc- MARK4 Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-5 and then treated with reference compound CTx-0294885 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Readout
Binding of compound to target kinase
Reference Compound IC50s
CompoundsIC50 (M)
CTx-02948851.903e-007
Screening Location
Malvern, USA
Reference compound IC50 for MARK4
Reference compound IC50 for MARK4