MTHFD2 Replication-related Assay for compound screening and profiling via quantification of enzyme activity.
Target Overview
CAT#
MTHFD2
Enzyme
Human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Synonyms
NMDMC, NAD-dependent methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase
Substrate
50 µM Folitixorin (5,10-Methylenetetrahydrofolic Acid), 500 µM NAD
Assay Properties
Reaction
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate+ NAD+ = 5,10-methenyltetrahydrofolate + NADH
End point measurement, Diaphorase/Resazurin coupled detection, Resorufin fluorescence measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| DS18561882 | 0.008 |
| DS44960156 | 1.34 |
Scientific Information
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Replication-related Assays
Reference compound IC50 for MTHFD2
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