Replication-related Assays

Reaction Biology currently offers the following services for replication-related assays.

  • Low scare, large scale, and HTS screening is available
  • Single-dose screening and IC50 profiling with 10 doses 
  • Ki Determination
  • Custom Assay development

List of Targets

Target Synonym UniProt Data
MTH1 NUDT1, 7,8-dihydro-8-oxoguanine triphosphatase Data sheet
MTHFD1 C1-THF synthase, Methylenetetrahydrofolate dehydrogenase, Epididymis secretory sperm binding protein, MTHFC, MTHFD Data sheet
MTHFD2 NMDMC, NAD-dependent methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase Data sheet

Assay Details

Assay Setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format. 

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20 µl of a 10 mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.