NEDP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
NEDP1
Enzyme
Human NEDP1
Synonym
Sentrin-specific protease 8, Deneddylase-1, NEDD8-specific protease 1,Protease cysteine 2, Sentrin/SUMO-specific protease SENP8, DEN1, PRSC2, FKSG8)
Substrate
Fluorogenic substrate [NEDD8-AMC], 100 nM
Assay Properties
Reaction
Cleavage of NEDD8 for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 6.24 |
| UIPase I | 78 |
| UI1 | 99 |
Scientific Information
Q96LD8 (http://www.uniprot.org/uniprot/Q96LD8)
Screening Location
Malvern, USA
Reference Compound IC50 for NEDP1
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