NEDP1 Ubiquitin-Proteasome Pathway Assay Service

NEDP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

NEDP1

Enzyme

Human NEDP1

Synonym

Sentrin-specific protease 8, Deneddylase-1, NEDD8-specific protease 1,Protease cysteine 2, Sentrin/SUMO-specific protease SENP8, DEN1, PRSC2, FKSG8)

Substrate

Fluorogenic substrate [NEDD8-AMC], 100 nM

Assay Properties
Reaction

Cleavage of NEDD8 for release of fluorescent AMC

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (µM)
UCHL1 I 6.24
UIPase I 78
UI1 99
Scientific Information

Q96LD8 (http://www.uniprot.org/uniprot/Q96LD8)

Screening Location

Malvern, USA

Reference Compound IC50 for NEDP1

Reference Compound IC50 for NEDP1