Activity-based PARP1 assay for compound screening and profiling via NAD/NADH-GloTM.
Target Overview
CAT#
PARP1
Enzyme
Human PARP1
Synonym
Poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1, Poly[ADP-ribose] synthase 1, ADPRT, PPOL
Scientific Information
P09874 (http://www.uniprot.org/uniprot/P09874)
Assay Properties
Substrate
Protein substrate, Core Histones, 0.01 mg/ml
Activated DNA, 10 µg/ml
NAD+ (Nicotinamide Adenine Dinucleotide), 500 nM
Reaction
Substrate + NAD+ = (ADP-D-ribosyl)-Substrate + Nicotinamide
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Olaparib (AZD2281) | 0.2 |
| Veliparib (ABT-888) | 3.1 |
| Niraparib (MK-4827) | 1.5 |
| PJ34 | 15.86 |
| NVP-TNKS656 | 51600 |
| MN-64 | 790 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Poly (ADP-ribose) Polymerase (PARP) Assay Services
Reference compound IC50 for PARP1
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