Poly (ADP-ribose) Polymerase (PARP) Assay Services

Poly (ADP-ribose) polymerase (PARP) is a family of proteins that transfer ADP-ribose units from NAD+ onto target nuclear proteins forming long branched Poly ADP-ribose chains. PARPs play a role in epigenetic regulation, for example, by poly ADP-ribosylation of histone substrates.

  • Gold standard assay format: radiometric activity assay
  • Low and large scale screening, as well as high-throughput screening options, are available
  • Custom assay development possible
  • PARP1 and PARP2 are produced in house and available for purchase

List of Targets

Target Synonyms Data
PARP1 PARP1 Data sheet
PARP2 ADPRT2 Data sheet
PARP5B TNKS2 Data sheet

Assay Details

Assay Format

 

Assay principle of PARP compound screening based on transfer of 32P-labelled ADP-ribose onto histones

Adenylate-NAD+ serves as co-factor for transfer of 32P-labelled ADP-ribose units onto histones which is quantified via scintillation counting.

Assay Setup

Setups: Single-dose screening in duplicates or IC50 value determination with 5 or 10 concentrations. Other screening formats are available upon request.

Controls: No inhibitor (DMSO vehicle) control and for every assay, one target-specific control compound is tested in 10-dose IC50 format. 

Turnaround time: 10 business days for standard projects. Expedited scheduling and data delivery can be arranged prior to the commencement of the studies.

Report: The raw data, % enzyme activity and control compound IC50 values will be reported in Excel format for single-dose assays. For IC50 orders, raw data, IC50 values, and curve fitting will be delivered in Excel format. Assay conditions, target, and substrate information are available upon request. Requirements for this information should be noted prior to the commencement of the study.

Screening facility: This assay is performed at our screening facility in Malvern, PA, US.

Compound requirements: In brief, for a standard project, 20ul of a 10mM DMSO stock or solid material is needed. Less material is needed for large scale screening. Please refer to our FAQs for information regarding compound preparation and shipping.