PARP2 Assay Service

Activity-based PARP2 assay for compound screening and profiling via NAD/NADH-GloTM.

Target Overview

CAT#

PARP2

Enzyme

Human PARP2

Synonym

Poly [ADP-ribose] polymerase 2, ADP-ribosyltransferase diphtheria toxin-like 2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthase 2, ADPRT2, ADPRTL2

Scientific Information

Q9UGN5 (http://www.uniprot.org/uniprot/Q9UGN5)

Assay Properties

Substrate

Protein substrate, Histone H3.3, 5 µM
Activated DNA, 10 µg/ml
NAD+ (Nicotinamide Adenine Dinucleotide), 500 nM

Reaction

Substrate + NAD+ = (ADP-D-ribosyl)-Substrate + Nicotinamide

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Olaparib (AZD2281) 0.6
Veliparib (ABT-888) 4.9
Niraparib (MK-4827) 6.6
NVP-TNKS656 30690
MN-64 3990

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Poly (ADP-ribose) Polymerase (PARP) Assay Services.

Reference compound inhibition for PARP2

Reference compound inhibition for PARP2