Activity-based PARP5B/TNKS2 assay for compound screening and profiling via NAD/NADH-GloTM.
Target Overview
CAT#
PARP5B/TNKS2
Enzyme
Human PARP5B/TNKS2
Synonym
Tankyrase-2, TANK2, Poly [ADP-ribose] polymerase 5B, ADP-ribosyltransferase diphtheria toxin-like 6, TRF1-interacting ankyrin-related ADP-ribose polymerase 2, Tankyrase II, Tankyrase-like protein, Tankyrase-related protein, TNKL
Scientific Information
Q9H2K2 (http://www.uniprot.org/uniprot/Q9H2K2)
Assay Properties
Substrate
Protein substrate, Histone H2A, 20 µM
Activated DNA, 10 µg/ml
NAD+ (Nicotinamide Adenine Dinucleotide), 500 nM
Reaction
Substrate + NAD+ = (ADP-D-ribosyl)-Substrate + Nicotinamide
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Olaparib (AZD2281) | 1330 |
| Veliparib (ABT-888) | 14760 |
| Niraparib (MK-4827) | 13420 |
| PJ34 | 910 |
| NVP-TNKS656 | 28 |
| MN64 | 11 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Poly (ADP-ribose) Polymerase (PARP) Assay Services.
Reference compound IC50 for PARP5B/TNKS2
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