PDE10A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE10A
Enzyme
Human PDE10A
Synonym
cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A
Scientific Information
Q9Y233 (http://www.uniprot.org/uniprot/Q9Y233)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 9860 |
| TC-E 5005 | 5.4 |
| Rolipram | >100000 |
| Methoxymethyl-IBMX | 13600 |
| Trequinsin | 12.2 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE10A
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