PDE1A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE1A
Enzyme
Human PDE1A
Synonym
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1A, 61 kDa Cam-PDE, hCam-1)
Scientific Information
P54750 (http://www.uniprot.org/uniprot/P54750)
Assay Properties
Substrate
0.2 µM cGMP, Ca-CaM
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Methoxyquinazoline | 738 |
| BAY 60-7550 | 14.6 |
| Sildenafil | 256 |
| IBMX | 1260 |
| ITI-214 | 0.0028 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE1A
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