PDE1B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE1B
Enzyme
Human PDE1B
Synonym
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1B, 63 kDa Cam-PDE, PDE1B1, PDES1B
Scientific Information
Q01064 (http://www.uniprot.org/uniprot/Q01064)
Assay Properties
Substrate
0.2 µM cGMP, Ca-CaM
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 20200 |
| Trequinsin | 5610 |
| Methoxymethyl IBMX | 11500 |
| BAY 60-7550 | 160 |
| ITI-214 | 0.062 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE1B
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