PDE1C Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE1C
Enzyme
Human PDE1 C
Synonym
Calcium/calmodulin-dependent 3′,5′-cyclic nucleotide phosphodiesterase 1C, hCam-3
Scientific Information
Q14123 (http://www.uniprot.org/uniprot/Q14123)
Assay Properties
Substrate
0.2 µM cGMP, Ca-CaM
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Methoxymethyl IBMX | 2030 |
| Trequinsin | 1500 |
| Sildenafil | 2260 |
| BAY 60-7550 | 29.7 |
| ITI-214 | 0.007 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE1C
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