PDE1C Phoshodiesterase Assay Service

PDE1C Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE1C

Enzyme

Human PDE1 C

Synonym

Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C, hCam-3

Substrate

1 µM cGMP, Ca-CaM

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 35,000
Methoxymethyl-IBMX 28,000
Roliptam >100,000
Methoxyquinazoline >100,000
Scientific Information

Q14123 (http://www.uniprot.org/uniprot/Q14123)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE1C

Reference Compound IC50 for PDE1C