PDE2A Phoshodiesterase Assay Service

PDE2A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE2A

Enzyme

Human PDE2A

Synonym

cGMP-dependent 3',5'-cyclic phosphodiesterase, Cyclic GMP-stimulated phosphodiesterase, cGS-PDE)

Substrate

1µM cGMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 7,600
Methoxymethyl-IBMX 7,900
Roliptam >50,000
Methoxyquinazoline >41,000
Scientific Information

O00408 (http://www.uniprot.org/uniprot/O00408)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE2A

Reference Compound IC50 for PDE2A