PDE2A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE2A
Enzyme
Human PDE2A
Synonym
cGMP-dependent 3′,5′-cyclic phosphodiesterase, Cyclic GMP-stimulated phosphodiesterase, cGS-PDE
Scientific Information
O00408 (http://www.uniprot.org/uniprot/O00408)
Assay Properties
Substrate
1µM cGMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Methoxymethyl IBMX | 6060 |
| BAY 60-7550 | 0.37 |
| Trequinsin | 161 |
| IBMX | 4230 |
| Dipyridamole | 3110 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE2A
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