PDE3A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE3A
Enzyme
Human PDE3A
Synonym
cGMP-inhibited 3′,5′-cyclic phosphodiesterase A, CGI-PDE A
Scientific Information
Q14432 (http://www.uniprot.org/uniprot/Q14432)
Assay Properties
Substrate
0.2 µM cGMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 864 |
| Cilostamide | 27.4 |
| Sildenafil | 44200 |
| Milrinone | 201 |
| Trequinsin | 0.03 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE3A
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