PDE3B Phoshodiesterase Assay Service

PDE3B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview

CAT#

PDE3B

Enzyme

Human PDE3B

Synonym

cGMP-inhibited 3′,5′-cyclic phosphodiesterase B, CGI-PDE B

Substrate

1 µM cAMP

Assay Properties

Reaction

Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 6,400
Methoxymethyl-IBMX >100,000
Roliptam >100,000
Methoxyquinazoline >100,000

Scientific Information

Q13370 (http://www.uniprot.org/uniprot/Q13370)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE3B

Reference Compound IC50 for PDE3B