PDE3B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE3B
Enzyme
Human PDE3B
Synonym
cGMP-inhibited 3′,5′-cyclic phosphodiesterase B, CGI-PDE B
Scientific Information
Q13370 (http://www.uniprot.org/uniprot/Q13370)
Assay Properties
Substrate
0.2 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 2100 |
| Cilostamide | 67.9 |
| Sildenafil | 22500 |
| Milrinone | 411 |
| Trequinsin | 0.05 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE3B
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