PDE4A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE4A
Enzyme
Human PDE4A
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 4A, DPDE2, PDE46
Scientific Information
P27815 (http://www.uniprot.org/uniprot/P27815)
Assay Properties
Substrate
1 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 23600 |
| RO-20-1724 | 644 |
| Roliptam | 1360 |
| Trequinsin | 112 |
| Cilostamide | >100000 |
| Milrinone | >100000 |
| Methoxyquinazoline | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE4A
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