PDE4B Phoshodiesterase Assay Service

PDE4B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview

CAT#

PDE4B

Enzyme

Human PDE4B

Synonym

cAMP-specific 3′,5′-cyclic phosphodiesterase 4B, DPDE4, PDE32

Substrate

1 µM cAMP

Assay Properties

Reaction

Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 12,900
Methoxymethyl-IBMX >50,000
Roliptam 630
Methoxyquinazoline 830

Scientific Information

Q07343 (http://www.uniprot.org/uniprot/Q07343)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE4B

Reference Compound IC50 for PDE4B