PDE4B Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE4B
Enzyme
Human PDE4B
Synonym
cAMP-specific 3',5'-cyclic phosphodiesterase 4B, DPDE4, PDE32
Substrate
1 µM cAMP
Assay Properties
Reaction
Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 12,900 |
| Methoxymethyl-IBMX | >50,000 |
| Roliptam | 630 |
| Methoxyquinazoline | 830 |
Scientific Information
Q07343 (http://www.uniprot.org/uniprot/Q07343)
Screening Location
Malvern, USA
Reference Compound IC50 for PDE4B
