PDE4C Phoshodiesterase Assay Service

PDE4C Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE4C

Enzyme

Human PDE4C

Synonym

cAMP-specific 3',5'-cyclic phosphodiesterase 4C, DPDE1, PDE21)

Substrate

1 µM cAMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 13,700
Methoxymethyl-IBMX >50,000
Roliptam 6,100
Methoxyquinazoline 12,700
Scientific Information

Q08493 (http://www.uniprot.org/uniprot/Q08493)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE4C

Reference Compound IC50 for PDE4C