PDE4C Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE4C
Enzyme
Human PDE4C
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 4C, DPDE1, PDE21
Scientific Information
Q08493 (http://www.uniprot.org/uniprot/Q08493)
Assay Properties
Substrate
1 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 50600 |
| RO-20-1724 | 2940 |
| Rolipram | 8640 |
| Trequinsin | 1700 |
| Methoxyquinazoline | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE4C
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