PDE4D2 Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#
PDE4D2
Enzyme
Human PDE4D2
Synonym
cAMP-specific 3′,5′-cyclic phosphodiesterase 4D2
Scientific Information
Q08499 (http://www.uniprot.org/uniprot/Q08499)
Assay Properties
Substrate
1 µM cAMP
Reaction
Nucleoside 3′,5′-cyclic phosphate + H₂O = nucleoside 5′-phosphate
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| IBMX | 2010 |
| RO-20-1724 | 547 |
| Rolipram | 151 |
| Trequinsin | 225 |
| Milrinone | 34100 |
| Cilostamide | >100000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website PDE Assay Services.
Reference Compound IC50 for PDE4D2
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