PDE4D Phoshodiesterase Assay Service

PDE4D Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE4D

Enzyme

Human PDE4D

Synonym

cAMP-specific 3',5'-cyclic phosphodiesterase 4D, DPDE3, PDE43)

Substrate

1 µM cAMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX 9,900
Methoxymethyl-IBMX >50,000
Roliptam 1,800
Methoxyquinazoline >50,000
Scientific Information

Q08499 (http://www.uniprot.org/uniprot/Q08499)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE4D

Reference Compound IC50 for PDE4D