PDE5A Phoshodiesterase Assay Service

PDE5A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE5A

Enzyme

Human PDE5A

Synonym

cGMP-specific 3',5'-cyclic phosphodiesterase, CGB-PDE)

Substrate

1 µM cAMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX >50,000
Methoxymethyl-IBMX >50,000
Roliptam >50,000
Methoxyquinazoline 7,900
Scientific Information

O76074 (http://www.uniprot.org/uniprot/O76074)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE5A

Reference Compound IC50 for PDE5A