PDE8A Phoshodiesterase Assay Service

PDE8A Phosphodiesterase Assay for compound screening and profiling via fluorescence polarization-based quantification of enzyme activity.
Target Overview
CAT#

PDE8A

Enzyme

Human PDE8A

Synonym

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Substrate

1 µM cAMP

Assay Properties
Reaction

Nucleoside 3',5'-cyclic phosphate + H₂O = nucleoside 5'-phosphate

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
IBMX >100,000
Methoxymethyl-IBMX >100,000
Roliptam >100,000
TC-E 5005 58,000
Scientific Information

O60658 (http://www.uniprot.org/uniprot/O60658)

Screening Location

Malvern, USA

Reference Compound IC50 for PDE8A

Reference Compound IC50 for PDE8A